Genotyping of human apolipoprotein E alleles by the new qualitative, microplate-based CASSI-detection assay.

A rapid, microplate SNP genotype assay for the leptinob allele.

A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptinob mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptinob genotyping is necessary. To date, gel-based assays have been used for genotyping. Using the Invader Plus assay for single...

Frequency of Apolipoprotein E Alleles in Iranian Populations

Investigation of Human Leukocyte Antigen (HLA) Class I and II Alleles and Hepatitis C Virus (HCV) Genotypes in Patients Infected with HCV

Hepatitis C virus (HCV) is a major public health problem with more than 130-180 million people infected worldwide. Several studies in different populations have reported the association of human leukocyte antigen (HLA) genotypes, and HCV viral load, and genotypes. The aim of this study was to investigate a possible association between HLA class I and II alleles in HCV-infected patients and heal...

Evaluation of a New Anti-HIV1/2 ELISA–HIV 1/2 REC Diagnostic Kit Based on E. coli Derived Soluble Recombinant Proteins: Ex-perience of an International Study

Background/Objective: Development of a new enzyme-linked immunosorbent assay (ELISA) for screening human blood serum and plasma for antibodies to human immunodeficiency virus type 1 (HIV1) and type 2 (HIV2) as HIV1/2REC ELISA diagnostic kit based on E. coli derived soluble recombinant proteins. Methods: Polypeptides corresponding to HIV1 gp41 and HIV2 gp36 immunodominant regions and HIV1 gag we...

A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...